Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 6. A typical example of a Vi/Vc neuron responsive to balloon distension of the parotid gland. ( A ) The administration of 0.5, 1.0, and 1.5 ml of air into the balloon causes graded neuronal spikes in response to parotid distention. PD parotid distention with balloon. ( B ) Peak frequency of neuronal responses to distension stimulation applied to the parotid gland (Reproduced with kind permission from Elsevier Japan: Ogawa et al., Brain Res., 2006, 1106:123–133).

Article Snippet: Sandoz, G., Thummler, S., Duprat, F., Feliciangeli, S., Vinh, J., Escoubas, P., Guy, N., Lazdunski, M., and Lesage, F. (2006) AKAP150, a switch to convert mechano-, pH- and arachidonic acid-sensitive TREK K(+) channels into open leak channels.

Techniques:

Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 7. Receptive fi elds of neurons in the C1/C2 and Vi/Vc regions following capsaicin administration into the parotid gland. The area of both low ( open circle ) and high ( fi lled circle ) threshold responses increases in C1/C2 neurons following capsaicin treatment ( A ), whereas no expansion of the receptive fi eld occurs in Vi/Vc neurons ( C ). Representative examples of the capsaicin effects on receptive fi eld sizes in a C1/C2 neuron ( B ) and Vi/Vc neuron ( D ) before and after capsaicin treatment. Low threshold areas responded to gentle touching with camel hair and noxious mechanical stimulation by small arterial clip ( solid areas ), and high threshold areas responded to only noxious mechanical stimulation ( shaded areas ) are indicated (Reproduced with kind permission from Elsevier Japan: Ogawa et al., Brain Res., 2006, 1106:123–133).

Article Snippet: Sandoz, G., Thummler, S., Duprat, F., Feliciangeli, S., Vinh, J., Escoubas, P., Guy, N., Lazdunski, M., and Lesage, F. (2006) AKAP150, a switch to convert mechano-, pH- and arachidonic acid-sensitive TREK K(+) channels into open leak channels.

Techniques: Gentle

Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 8. Peak fi ring frequency following graded mechanical stimulation of the cutaneous receptive fi eld. Capsaicin administration into the parotid gland signifi cantly increases mechanically evoked responses of C1/C2 ( open circle ), but not Vi/Vc ( fi lled circle ) neurons. Peak fi ring frequency in control stimulation trials is not different between Vi/Vc and C1/C2 neurons. Stimulus intensities to the receptive fi eld are indicated at the top left corner of each panel (A-F). # P < 0.05, ## P < 0.01 versus pre-capsaicin controls. * P < 0.05, ** P < 0.01 versus Vi/Vc neurons (Reproduced with kind permission from Elsevier Japan: Ogawa et al., Brain Res., 2006, 1106:123–133).

Article Snippet: Sandoz, G., Thummler, S., Duprat, F., Feliciangeli, S., Vinh, J., Escoubas, P., Guy, N., Lazdunski, M., and Lesage, F. (2006) AKAP150, a switch to convert mechano-, pH- and arachidonic acid-sensitive TREK K(+) channels into open leak channels.

Techniques: Control

Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 9. Peak fi ring frequency of Vi/Vc neurons ( fi lled circle ) and C1/C2 neurons ( open circle ) following graded heat stimulation of the receptive fi eld after capsaicin administration into the parotid gland. Unlike mechanically evoked responses, heat-evoked responses are not affected by capsaicin administrations. Stimulus intensities are indicated at the top left corner of each panel (A-D) (Reproduced with kind permission from Elsevier Japan: Ogawa et al., Brain Res., 2006, 1106:123–133).

Article Snippet: Sandoz, G., Thummler, S., Duprat, F., Feliciangeli, S., Vinh, J., Escoubas, P., Guy, N., Lazdunski, M., and Lesage, F. (2006) AKAP150, a switch to convert mechano-, pH- and arachidonic acid-sensitive TREK K(+) channels into open leak channels.

Techniques:

Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 10. Changes in peak fi ring frequency of Vi/Vc neurons ( fi lled circle ) and C1/C2 neurons ( open circle ) following graded cold stimulation of the receptive fi elds after capsaicin administration into the parotid gland. Stimulus intensities are indicated at the top left corner of each panel (A-C). The reduction in cold stimulation evoked activity in Vi/Vc neu- rons reaches a statistical signifi cance at 20°C following capsaicin administration. The evoked responses of C1/C2 neurons are signifi cantly larger than those of Vi/Vc neurons following capsaicin administration. # P < 0.05 versus pre-capsaicin controls. * P < 0.05 ver- sus Vi/Vc neurons (Reproduced with kind permission from Elsevier Japan: Ogawa et al., Brain Res., 2006, 1106:123–133).

Article Snippet: Sandoz, G., Thummler, S., Duprat, F., Feliciangeli, S., Vinh, J., Escoubas, P., Guy, N., Lazdunski, M., and Lesage, F. (2006) AKAP150, a switch to convert mechano-, pH- and arachidonic acid-sensitive TREK K(+) channels into open leak channels.

Techniques: Activity Assay